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MOLECULAR BIOLOGY: WORKING WITH PROTEINS 1. Chick Embryo ExtractPURIFICATION: EXTRACT FROM CELLS
2. Cytostatic Factor (CSF) Extract Preparation for Spindle Assembly
3. Glass Bead Lysis of Yeast
4. HeLa Nuclear Extract Preparation
5. Isolation and Extraction of Protein from Mammary Epithelial Cells Grown in 3D Engelbreth-Holm-Swarm (EHS) Extracellular Matrix Cultures
6. Isolation and Storage of Rat Serum Conjugase for Tissue Folate Analysis
7. Large Scale Preparation and Purification by Ammonium Sulfate Precipitation of Proteins from Yeast Whole Cell Extract
8. Preparation of a Nuclear Protein Extract from Drosophila Embryos
9. Preparation of Chick Embryo Extract
10. Preparation of Clear Lysates from Bacteria
11. Preparation of Embryonic Extract from Chicken Embryos
12. Processing of Microdissected Tissue for Molecular Analysis: DNA-based Studies, RNA-based Studies, and Protein-based Studies
13. Protein Dialysis
14. Protein Gel Extracts from Worms for Analysis by SDS-PAGE
15. Purification Using Biogel P-10™ Spin Columns
16. Rapid Preparation of Protein Samples from Mammalian Cells for Western Blotting Analysis
17. Rapid Preparation of Whole Cell Yeast Extract
18. Rapid Yeast Protein Preparation
19. Rapid Yeast Protein Preparation for SDS-PAGE and Western Analysis
20. SDS-PAGE Sample Preparation of Adenovirus Infected Cells
21. Small Scale Preparation of Nuclear Extracts from HeLa Cells
22. Whole Cell Yeast Extract
23. Xenopus Egg CSF Extract Preparation
24. Yeast Mini Whole Cell Extract for Western Analysis Using Glass Beads
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